Preliminary analysis of CD41 monoclonal antibody failed to establish a mouse model of chronic immune thrombocytopenia

ZHANG Jun; ZHU Ruifang; LYU Yaru; LI Ruolan; ZHANG Shuwen; LI Na; SONG Kai; ZENG Jinghui; HAN Shifan

doi:10.12102/j.issn.1009-6493.2024.07.002

Abstract：Objective:CD41 monoclonal antibody(MWReg30)was injected into Balb/c mice at different times and doses to investigate whether this method can construct a stable model of chronic Immune thrombocytopenia(ITP).After the modeling,the extract of Codonopsis pilosula was used to intervene the mice,in order to clarify its adjustment effect on abnormal platelet count of model mice.Methods:Firstly,chronic ITP model was established in Balb/c mice using MWReg30,and the effect of Codonopsis pilosula's extract on the model mice was investigated after antibody injection.Subsequently,different groups of mice were given a second injection and a double dose of MWReg30 to determine whether the increase in the number of injections and dose was conducive to the establishment of chronic ITP model.Results:It was confirmed that the method of injecting MWReg30 into mice could not keep the platelet number of Balb/c mice at a low level for a long time,so the method could not establish a stable chronic ITP model,and it was difficult to achieve this purpose by increasing the number and dose of antibody injection.However,second and double dose injections of MWReg30 resulted platelet count antibody rebound to higher levels within 7 days and 14 days after the end of the injection,and the low dose of Codonopsis pilosula extract could decrease the abnormal platelet count of the model mice.Conclusion:The intraperitoneal injection of MWReg30 in mice could not reproduce stable chronic ITP models,and whether this method can be an ideal way to construct chronic ITP models remains to be further confirmed.Increasing the injection dose of MWReg30 can prolong the maintenance time of low platelet count in model mice,and low dose of Codonopsis pilosula extract may correct the abnormal elevated platelet count in model mice.