Experimental study on influence of low temperature enteral feeding on rats with gastric ischemia reperfusion injury
Dai Li
Luo Lina
Zeng Qiuxia
Abstract：Objective:To probe into the influence of low temperature enteral feeding on rats with gastric ischemia reperfusion injury (GI RI)and its main mechanisms.Methods:A total of 40 male Sprague Dawley(SD)rats were randomly divided into four groups according to enteral feeding temperature:10 ℃,24 ℃,32 ℃ and 40 ℃(n=10 per group).Clamping the celiac artery for 30 min to lead gastric ischemia and then to removed the clip to make GI RI model.At 2 h later after reperfusion,all rats received enteral feeding .When 48 h later after the reperfu-sion,gastric mucosal injury index was counted grossly.The expression of tumor necrosis factor α(TNF α)of gastric mucosal cell was detected by immunohistochemistry.And the plasma TNF a was detected with enzyme linked immunosorbent assay(ELISA).Results:The gastric mucosal injury index and positive expression of TNF αin 10 ℃ group were significantly lower than that in the other groups(P <0.05 or P <0.01).Meanwhile,those were positively correlated with enteral feeding temperature(P <0.01 ).There was no statistically difference in terms of plasma TNF α content among four groups(P >0.05).Conclusion:The results suggested that 10 ℃enteral feeding show a gastroprotective effect for the ischemia reperfusion injury in rats,The mechanism may be related to low temperature inhibiting the body’s inflammatory response over expression.
Keywords：enteral feedingtemperatureratgastric ischemia reperfusionacute gastric mucosal lesion
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1575-1577 )