Preliminary study on influence of hot and cold care stimulus on forming ability of human cervical cancer HeLa cells clone and its mechanism
Luo Yu
Wang Xianyuan
Abstract：Objective:To probe into the influence of hot and cold care stimulus on forming ability of human cervi-cal cancer HeLa cells clone and its mechanism,so as to explore the new action mechanism of hot and cold care promote the improvement of clinical nursing level.Methods:HeLa cells were cultured by routine use of DMEM high glucose medium.According the concentration of 100~300 cells per hole,they were inoculated in 6 hole plate,cell cloning experiments were carried out in different culture temperature circumstances;the total RNA of the same batch of HeLa cells was extracted which grew in different temperatures,the cDNA obtained in the re-verse transcription reaction was taken as a template,the fluorescent quantitative analysis was implemented for expression level of pan QKI mRNA under different temperature environment.Results:The high ambient tem-perature can enhance the colony formation ability of HeLa cells and inhibit the pan QKI mRNA expression;but the lower ambient temperature inhibited the cell clone formation,at the same time,the expression level of mRNA was significantly increased in pan QKI.Conclusion:Temperature intervention can affect cloning ability of human cervical cancer HeLa cells.The changes of pan QKI expression in different temperature environ-ments may be an important reason of differences of HeLa cell clone forming ability.The efficacy and mechanism of temperature intervention are worth for in depth inquiry.
Keywords：hot and cold carecervical cancerHeLa cellcell clonenursing intervention
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 18-21 )