Study on influence of hypoxia intervention on growth and survival of HeLa cel ls in human cervical carcinoma
Luo Yu
Wang Xianyuan
Abstract：Obj ective:To study the influence of hypoxia nursing interven-tion on cycle and survival of HeLa cells in human cervical carcinoma,in order to provide the basis for formulating new measures of clinical oncol-ogy nursing.Methods:The HeLa cells were cultured in conventional DMEM high glucose medium;after 1 000μM Two cobalt chloride storage liquid were prepared,it respectively constructed two cobalt chloride con-centration of 50μM,100μM and 200μM to simulate mild hypoxia,mod-erate hypoxia and severe hypoxia environment.After incubating for 24 h at 3 7 ℃,then the cell cycle,cell survival rate and clone forming ability were measured according to the flow cytometry detection program.Re-sults:Severe hypoxia can obviously induce the percentage of G1 phase cells in HeLa cells decent,but the proportion of cells in G2/M phase in-creased significantly;severe hypoxia can lead to the survival rate of HeLa cell decent;the severity of hypoxia was positively correlated with the de-gree of inhibition of HeLa cell clone forming ability.Conclusion:Hypoxia can seriously affect the growth and survival of HeLa cells.According to this,the targeted new measures development and clinical research could be carried out in nursing intervention,in order to carry out more effec-tively personalized nursing for patients with cervical cancer.
Keywords：cervical cancerhypoxiaHeLa cellcell interventioncell sur-vival
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3867-3870 )