Experimental study on establ ishment of pressure ulcers rat model and mold

Li Xiuyun; Cao Juan; Tang Jing

doi:10.3969/j.issn.10096493.2014.19.012

Abstract：Obj ective:To establish a rat model of pressure ulcers by using a simple mold tool,evaluate its characteristics and put it applied in basic re-search and clinical treatment research of pressure ulcers.Methods:A total of 10 Wistar rats were randomly divided into control group(n=2)and model group (n=8).The rats in control group did not receive any pressure;the rats in model group were given 30 kPa pressure on the bone protuberance close to knee and three ischemia reperfusion cycles for every day,lasting for two days.No any treatment was done for them in three days after that,and all rats were sacrificed on the fifth day.The skin integrity and color changes in pressed parts in rats were observed by naked eye and the epidermis and mus-cle tissue of pressure areas were observed under light microscope.Results:Rats in control group didn’t have any changes in skin and muscle tissue;skin ulceration occurred in rats in model group,and damage gradually extended from the epidermis,the dermis to the muscle layer.Conclusion:Pressure sores model established by the pressurized mold,histological and pathological changes of pressure sores are consistent to clinical pressure sores,and the model has stability and repeatability.The mold is easy to use,and it gives pressure precisely.