Determination of trivalent chromium and hexavalent chromium in feed additive calcium hydrogen phosphate
ZHANG Haoran
XU Ting
TAO Yujie
HUA Xianhui
HUANG Jiaying
Abstract：This study aimed to establish a method using high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry(HPLC-ICP-MS)for the determination of trivalent chromium[Cr(Ⅲ)]and hexavalent chromium[Cr(Ⅵ)]in the feed additive calcium hydrogen phosphate.Cr(Ⅵ)was extracted from the samples using an alkaline solution,while Cr(Ⅲ)was extracted using a dilute nitric acid solution.Chromatographic separation was performed on an AS7 anion exchange column(10 μm,4 mm×250 mm)with isocratic elution using a 100 mmol/L ammonium nitrate solution as the mobile phase.The results showed good linearity for both Cr(Ⅲ)and Cr(Ⅵ)within the range of 0～100 μg/L,with correlation coefficients greater than 0.999.Method validation demonstrated that for Cr(Ⅲ),the limit of detection was 0.1 mg/kg,the limit of quantification was 0.4 mg/kg,the recovery rate ranged from 90.6%to 97.9%,and the relative standard deviation(RSD)was between 3.6%and 5.2%.For Cr(Ⅵ),the limit of detection was 0.2 mg/kg,the limit of quantification was 0.4 mg/kg,the recovery rate ranged from 92.8%to 107.5%,and the RSD was between 4.5%and 8.4%.The study shows that the established method is simple,efficient,accurate,and reliable,and is suitable for the quantitative analysis of different forms of chromium in the feed additive calcium hydrogen phosphate.
Keywords：calcium hydrogen phosphateinductively coupled plasma mass spectrometrytrivalent chromiumhexavalent chromiumchromium speciation
Publication Date：2026-01-28
Online Publishing Date：2026-03-20(First online date of this platform, not the publication date of the document)
Pages：5( 113-117 )