Effects of fermentation time on fermentation quality and microbial diversity of sugarcane bagasse

HUANG Yali; LAN Bishi; ZHOU Junhua; HOU Xiaolu; ZHOU Xiaoqing; ZHOU Zhiyang; PENG Xiayun; LU Jianqin

doi:10.13557/j.cnki.issn1002-2813.2026.01.021

Abstract：This experiment aimed to investigate the effects of fermentation time on the fermentation quality and microbial diversity of sugarcane bagasse and to determine the optimal fermentation duration.Sugarcane bagasse was used as the raw material for natural fermentation.Samples were collected at 0(group D0),5(group D5),15(group D15),30(group D30),45(group D45),60(group D60),and 90 days(group D90)of fermentation to analyze the nutritional components,fermentation parameters,and microbial diversity.The results showed that the crude protein(CP)content in group D30 was significantly higher than the other groups(P＜0.05).The concentrations of dry matter(DM),crude fiber(CF),neutral detergent fiber(NDF),and acid detergent fiber(ADF)in group D0 were the highest.The lactic acid(LA)content in groups D0,D5,and D15 was significantly higher than the other groups(P＜0.05).The acetic acid(AA)content in groups D45,D60,and D90 was significantly higher than the other groups(P＜0.05).The propionic acid(PA)content in groups D30,D45,D60,and D90 was significantly higher than the other groups(P＜0.05).The butyric acid(BA)content in group D30 was significantly higher than the other groups except for group D45(P＜0.05).The dominant bacterial phyla in groups D5 to D90 were Firmicutes,Proteobacteria,and Actinobacteria,and the dominant bacterial genus were Acinetobacter.Caproiciproducens,Glutamicibacter,and Bifidobacterium were the dominant genera in groups D45,D60,and D90.Correlation analysis indicated that Lactococcus and Corynebacterium were negatively correlated with CF,ADF,and NDF.Aerococcus was positively correlated with LA,Caproiciproducens was positively correlated with AA and PA,and Bifidobacterium was positively correlated with PA.The study shows that the suitable fermentation time for sugarcane bagasse is 30 days.