Study on enhancement of pseudorabies vaccine immune efficacy by Lonicera japonica polysaccharides

WU Jiale; WANG Lu; SHU Ruiteng; XU Hao; ZHANG Yu; LIU Chang; LI Lei; CAO Mixia

doi:10.13557/j.cnki.issn1002-2813.2025.24.016

Abstract：This experiment aimed to investigate the immunomodulatory effects of Lonicera japonica polysaccharides(LJP)on mice immunized with pseudorabies vaccine.A total of 180 SPF-grade mice were randomly divided into six groups,with 15 replicates per group and two mice per replicate.The blank control group(group BC)was not immunized and did not receive LJP,the vaccine control group(group VC)was immunized but did not receive LJP,the Astragalus polysaccharide group(group APS)was immunized and administered 600 mg/kg Astragalus polysaccharide,the LJP low-dose group(group LJP-L)was immunized and administered 200 mg/kg LJP,the LJP medium-dose group(group LJP-M)was immunized and administered 400 mg/kg LJP,and the LJP high-dose group(group LJP-H)was immunized and administered 800 mg/kg LJP.The pre-test period was 2 days,and the formal test period was 32 days.The results showed that compared with group VC,the spleen and liver indexes in group LJP-M and the liver index in group LJP-H were significantly increased(P<0.05).In the group LJP-M,the serum levels of immunoglobulin G(IgG),immunoglobulin G1 subtype(IgG1),and immunoglobulin G2a subtype(IgG2a)antibody were extremely elevated(P<0.01),and the level of immunoglobulin G2b subtype(IgG2b)antibody was significantly increased(P<0.05).In the group LJP-H,IgG and IgG2a antibody levels were significantly increased(P<0.05),and IgG1 and IgG2b antibody levels were extremely increased(P<0.01).In the group LJP-L,the IgG1 antibody level was extremely increased(P<0.01).The levels of interferon-γ(IFN-γ),interleukin-2(IL-2),and interleukin-4(IL-4)in the serum of the group LJP-M were significantly increased(P<0.05),while the level of interleukin-10(IL-10)was extremely increased(P<0.01),the levels of IL-2 and IL-4 in the group LJP-L were significantly increased(P<0.05),and the level of IL-10 was extremely increased(P<0.01),the level of IL-4 in the group LJP-H was extremely increased(P<0.01),and the level of IL-10 was significantly increased(P<0.05).The study shows that LJP can significantly enhance the immune efficacy of the pseudorabies vaccine by synergistically inducing a mixed type 1 helper T cell/type 2 helper T cell(Th1/Th2)immune response,with the addition of 400 mg/kg LJP showing a better effect.