Study on process optimization of enzyme-assisted aqueous extraction of Arthrospira platensis polysaccharides and their in vivo antioxidant activity
YU Xiaoli
ZHOU Shumian
YU Meiling
WANG Qiuhua
SHI Jun
HU Tingjun
Abstract：This experiment aimed to optimize the enzyme-assisted aqueous extraction process of Arthrospira platensis polysaccharides(PAP)and evaluate their in vivo antioxidant activity.The optimal extraction method for PAP was identified by comparing different techniques.Single-factor tests combined with response surface methodology were employed to optimize the enzyme-assisted aqueous extraction process,and the antioxidant activity was assessed via a mouse model.The results showed that the optimal extraction method was enzyme-assisted aqueous extraction,with the best parameters being:Enzyme hydrolysis temperature of 50℃,extraction time 2.5 hours,and a liquid-to-material ratio of 40 mL/g.Under these conditions,the PAP extraction yield was 17.12%,which was close to the predicted value of 17.42%.Compared with the blank group,the activities of inducible nitric oxide synthase(iNOS)and catalase(CAT)in the serum of mice infected with porcine pseudorabies virus(PRV)were significantly increased(P<0.05),and the content of malondialdehyde(MDA)was significantly reduced(P<0.05).Compared with the PRV group,serum activities of CAT and glutathione peroxidase(GSH-Px)in PRV+PAP low-dose,medium-dose,and high-dose groups were significantly increased(P<0.05),and iNOS activity was significantly decreased(P<0.05).The MDA content in the serum of the PRV+PAP high-dose group was significantly decreased(P<0.05),and the activity of superoxide dismutase(SOD)in the serum of the PRV+PAP medium dose and high-dose groups was significantly increased(P<0.05).The study shows that the PAP extracted in this experiment has strong in vivo antioxidant activity,which can provide a reference for the development and application of PAP as an antioxidant additive in animal production.
Keywords：Arthrospira platensis polysaccharideenzyme-assisted aqueous extractionantioxidant activityresponse surface methodology
Publication Date：2025-12-28
Online Publishing Date：2026-01-31(First online date of this platform, not the publication date of the document)
Pages：6( 73-78 )