Extraction,composition analysis,and antioxidant activity study of Lycopus lucidus Turcz.oligosaccharides
YIN Kangning
ZHAO Xingwang
WANG Jing
YANG Zhijuan
WU Shaojie
Abstract：This experiment aimed to investigate the composition,structure,and biological activity of saccharide components from Lycopus lucidus Turcz..The enzymatic hydrolysis extraction process was optimized using single-factor experiment and response surface methodology to prepare crude saccharides.The structure of the saccharides was systematically characterized by high-performance gel permeation chromatography(HP-GPC),electrospray ionization high-resolution mass spectrometry(ESI-HRMS),and acid hydrolysis-ion chromatography.The results showed that under the conditions of enzymatic hydrolysis pH value of 6.50,enzymatic hydrolysis temperature of 52℃,and enzyme concentration of 2.80%,the content in the crude saccharides extract of Lycopus lucidus Turcz.was 175.26 mg/L.After purification by diethylaminoethyl cellulose-52(DEAE-52)column chromatography,the crude saccharides extract obtained the water-eluted polysaccharide fraction-1(LLP-1)and the low-salt-eluted polysaccharide fraction-2(LLP-2),with LLP-1 accounting for 88.3%of the total eluted components.Structural analysis revealed that LLP-1 was an oligosaccharide mixture with a degree of polymerization(DP)ranging from 1 to 13,of which oligosaccharides with DP 3 to 6 accounted for 85%,and no high-molecular-weight polysaccharides were detected.The monosaccharide composition of LLP-1 was fructose∶glucose∶galactose=8.10∶2.52∶1.00,infering that LLP-1 belongs to the raffinose family of oligosaccharides.LLP-1 had excellent in vitro antioxidant capacity and could dose dependently inhibit lipopolysaccharide(LPS)-induced oxidative stress in zebrafish embryos within a concentration range of≤200 mg/L.The study indicates that the experimental results can provide a reference for the application and development of Lycopus lucidus Turcz.as a feed additive in livestock breeding.
Keywords：Lycopus lucidus Turcz.oligosaccharidesextractioncharacterizationantioxidant activity
Publication Date：2025-12-16
Online Publishing Date：2026-01-31(First online date of this platform, not the publication date of the document)
Pages：7( 109-115 )