Optimization of extraction process for total flavonoids from Callistephus chinensis by response surface methodology and analysis of their antioxidant activity
HE Fan
CHEN Simin
HUANG Huiling
PENG Lingyu
LI Chengmin
LIAO Yaqing
JIANG Lichun
Abstract：This experiment aimed to optimize the extraction process of total flavonoids from Callistephus chinensis and explore its antioxidant properties.Using Callistephus chinensis as raw material,ultrasound-microwave assisted extraction(UMAE)combined with response surface methodology was used for process optimization,and the in vitro antioxidant activity was evaluated.The results showed that the optimal extraction conditions determined through single-factor tests and response surface experiment were as follows:Ethanol concentration 70％,liquid to material ratio 50 mL/g,extraction time 40 min,extraction temperature 40 ℃,and microwave power 400 W.Under these conditions,the extraction yield of total flavonoids reached 10.82％,with a relative error of only 0.08％compared to the model-predicted value(10.90％),indicating a good model fit.In vitro antioxidant tests demonstrated that the total flavonoids from Callistephus chinensis exhibited scavenging effects on 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals,hydroxyl free radicals,and sodium nitrite(NaNO2),with scavenging rates of 82.38％,58.76％,and 63.86％at optimal concentrations,respectively.The scavenging efficacy followed the order:DPPH free radicals＞NaNO2＞hydroxyl free radicals,and the scavenging effect showed a significant concentration dependence.The study shows that the optimized UMAE process significantly improves the extraction yield of total flavonoids from Callistephus chinensis,and total flavonoids of Callistephus chinensis have good in vitro antioxidant activity.
Keywords：Callistephus chinensistotal flavonoidsresponse surface methodologyultrasound-microwave assisted extractionantioxidant activity
Publication Date：2025-11-28
Online Publishing Date：2026-01-23(First online date of this platform, not the publication date of the document)
Pages：7( 104-110 )