Study on effect of carnosic acid in alleviating oxidative stress damage in bovine mammary epithelial cells

LU Jiaqi; ZHOU Jinhua; DU Jiaxuan; QU Xiaoxiao; KONG Zhiwei

doi:10.13557/j.cnki.issn1002-2813.2025.22.017

Abstract：This experiment aimed to investigate the intervention effect of carnosic acid(CA)on hydrogen peroxide(H2O2)-induced oxidative damage in bovine mammary epithelial cells(BMECs).The experiment established an oxidative stress model of BMECs induced by H2O2,and used techniques such as enzyme-linked immunosorbent assay(ELISA),fluorescence microscopic imaging,and real-time fluorescence quantitative PCR(RT-qPCR)to detect oxidative stress-related indicators.The results showed that treatment with 800 μmol/L H2O2 for 24 hours inhibited the survival of BMECs.However,after pretreatment with 12 μmol/L CA for 12 hours and then stimulation with 800 μmol/L H2O2 for 24 hours,the cell survival rate significantly increased.Compared with the control group,H2O2 treatment group were significantly increased the levels of reactive oxygen species(ROS)and malondialdehyde(MDA),as well as the mRNA expression level of Caspase3(P＜0.05),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the mRNA expression level of nuclear factor erythroid 2-related factor 2(Nrf2)were significantly decreased(P＜0.05).Compared with the H2O2 treatment group,CA pretreatment group significantly reduced the levels of ROS and MDA,Bcl-2-associated X protein/B-cell lymphoma/leukemia-2(Bax/Bcl-2)ratio,and the mRNA expression level of Caspase3(P＜0.05),while the activities of SOD and GSH-Px,as well as the mRNA expression levels of Nrf2,heme oxygenase-1(HO-1),and NAD(P)H quinone dehydrogenase 1(NQO1)were significantly increased(P＜0.05).The study shows that pretreatment with 12 μmol/L CA for 12 hours can effectively alleviate H2O2-induced oxidative stress damage in BMECs.