Effects of lysophosphatidylcholine on growth performance,serum biochemical indicators,liver morphology,and intestinal health in Micropterus salmoides fed low-fat diet

WEI Yuqiang; LIU Xiaochuan; BI Yulin; ZHAO Jianguo; ZHOU Zhihao; XU Qiyou

doi:10.13557/j.cnki.issn1002-2813.2025.18.013

Abstract：The experiment aimed to investigate the effects of dietary supplementation with lysophosphatidylcholine(LPC)in low-fat diets on the growth performance,serum biochemical indicators,liver morphology,and intestinal health of Micropterus salmoides.A total of 270 Micropterus salmoides with an initial body weight of(44.51±0.36)g were randomly divided into three groups,with three replicates per group,and 30 fish per replicate.The control group was fed a basal diet containing 12.05％crude fat,group LL1 was fed a diet of 11.55％crude fat supplemented with 0.05％LPC,and group LL2 was fed a diet of 11.05％crude fat supplemented with 0.10％LPC.The pre-test period was 14 days and the formal test period was 56 days.The results showed that compared with the control group,the weight gain rate(WGR)was significantly increased(P＜0.05)and the hepatosomatic index(HSI)was significantly decreased(P＜0.05)in both groups LL1 and LL2.The feed conversion ratio(FCR)was significantly reduced in group LL1(P＜0.05),while group LL2 showed an increasing trend without significance(P＞0.05).Serum total cholesterol(TC)content was significantly lower in group LL2(P＜0.05),and serum triglyceride(TG)content was significantly reduced in both groups LL1 and LL2(P＜0.05).High-density lipoprotein cholesterol(HDL-C)content was significantly increased in group LL1(P＜0.05),while group LL2 showed an increasing trend without significance(P＞0.05).The α-amylase activity was significantly higher in group LL2 than in the control group(P＜0.05).Villus height(VH)in the intestine was significantly improved in both groups LL1 and LL2(P＜0.05),and the number and size of hepatic vacuoles and the number of nuclei were significantly improved.The study indicates that supplementing LPC in reduced-fat diets effectively promotes growth,improves lipid metabolism and intestinal morphology,and reduces hepatic fat accumulation in Micropterus salmoides.Among them,adding 0.10％LPC while reducing fat levels by 1.00％has the best effect.