Study on enzymatic hydrolysis-assisted extraction of total ginkgolic acid from Ginkgo biloba exocarp
DU Xiaojing
ZHAO Gangying
CHEN Yi
LIANG Hao
Abstract：The experiment aimed to investigate the optimal process parameters for enzymatic hydrolysis-assisted ultrasonic extraction of total ginkgolic acid from Ginkgo biloba exocarp.Through single-factor experiments combined with Plackett-Burman(PB)tests,three main factors significantly affecting the total ginkgolic acid extraction rate were identified:The enzyme dosage,the enzymolysis time and the enzymolysis temperature,and combined with Box-Behnken response surface methodology to optimize the parameters.The results showed that when the extraction process parameters were as follows:Liquid pectinase dosage of 50 mg/g,enzymatic hydrolysis time of one hour,enzymatic hydrolysis temperature of 40 ℃,pH value of 5.0,ultrasonic time of 5 min,ultrasonic temperature of 40 ℃,and methanol concentration of 75％,the actual extraction rate of total ginkgolic acids was 9.05％.The study indicates that the method of enzymatic hydrolysis-assisted ultrasonic extraction of total ginkgolic acids from Ginkgo biloba exocarp is feasible.
Keywords：Ginkgo biloba exocarptotal ginkgolic acidsenzyme assisted-ultrasonic extractionPlackett-Burman designresponse surface methodology
Publication Date：2025-09-16
Online Publishing Date：2025-11-11(First online date of this platform, not the publication date of the document)
Pages：6( 85-90 )