Isolation and identification of Clostridium from whole-plant corn silage and analysis of their metabolites
YANG Fan
LÜ Jiaying
FU Dongqing
MA Chunhui
Abstract：The experiment aimed to investigate the species of Clostridium in whole-plant corn silage and the metabolics they produced.Taking Clostridium was isolated from whole-plant corn silage as the research object,through strain enrichment culture,isolation and purification,16S rDNA sequence determination,and the analysis of its physiology,biochemical and metabolic products.The results showed that one strain of Clostridium beijerinckii SHZ-8 was isolated and identified.The optimal growth pH value was 7.0 and the optimal growth temperature was 30 ℃.Decamethylcyclopentasiloxane was a potential metabolic indicator of it.The study indicates that strain SHZ-8 is a mesophilic and neutral bacterium,and the production of butyric acid and decamethylcyclopentasiloxane is an early warning signal of silage spoilage.
Keywords：whole-plant corn silageClostridiumgrowth patternmetabolites
Publication Date：2025-09-16
Online Publishing Date：2025-11-11(First online date of this platform, not the publication date of the document)
Pages：7( 65-71 )