Effects of perfluoro hexane sulfonic acid(PFHxS)exposure on embryo development and energy metabolism of zebrafish
BAI Shuangqian
LI Tingyin
BI Baoliang
HU Qing
Abstract：The experiment aimed to investigate the effects of perfluoro hexane sulfonic acid(PFHxS)exposure on embryo development and energy metabolism of zebrafish.A total of 10 groups of zebrafish parents(female:male ratio 1∶1)were selected for natural spawning and fertilization.Embryos were collected and randomly distributed into 12 petri dishes(approximately 500 embryos per dish).Four PFHxS exposure groups were set up,namely 0(control),1,10 and 100 μmol/L,with three replicates in each group.Embryo samples were collected at 72 and 120 hours post fertilization.The results showed that compared with the control group,the body length of zebrafish larvae in the 1 μmol/L group and the 100 μmol/L group was significantly reduced(P＜0.05),and the heart rate increased.At 72 hours post fertilization,the total cholesterol(TC)and triglyceride(TG)concentrations in the 10 μmol/L group and the 100 μmol/L group were significantly higher than those in the control group(P＜0.05).At 120 hours post fertilization,TG content was significantly higher in the 100 μmol/L group than in the 1 μmol/L group(P＜0.05).PFHxS exposure upregulated the expression levels of genes including carnitine palmitoyltransferase 1AB(cpt1ab),peroxisome proliferator-activated receptor alpha a(pparaa),nuclear factor kappa B subunit 2(nfκb2),lysozyme(lyz),thyroid-stimulating hormone beta subunit A(tshba),thyroid-stimulating hormone receptor(tshr),and thyroid hormone receptor beta(thrb).The expression level of the solute carrier family 2 member 12(slc2a12)gene in the 1 μmol/L group was significantly lower than in the control group(P＜0.05).The study indicates that PFHxS can affect the normal growth and development of zebrafish embryos by interfering with the homeostasis of energy metabolism,immune defense system and the expression of genes related to the hypothalamic-pituitary-thyroid(HPT)axis.
Keywords：zebrafishPFHxSembryo developmentenergy metabolism
Publication Date：2025-09-16
Online Publishing Date：2025-11-11(First online date of this platform, not the publication date of the document)
Pages：5( 48-52 )