Optimization of extraction process for polysaccharides from Castanea mollissima endocarp and analysis of their antioxidant function and anti-inflammatory activities
ZHANG Tingting
XU Yuan
YANG Miaoshuo
LIU Tingwei
LIU Minjie
ZHANG Bing
BI Jingjing
Abstract：The experiment aimed to optimize the extraction process of polysaccharides from the Castanea mollissima endocarp and explore their antioxidant function and anti-inflammatory activity.The extraction process of polysaccharides from the Castanea mollissima endocarp was optimized by single-factor experiments and response surface methodology.The antioxidant function was evaluated by determining the scavenging rates of 2,2-diphenyl-l-picrylhydrazyl(DPPH)and hydroxyl radicals,and the anti-inflammatory activity was evaluated by constructing a lipopolysaccharide(LPS)-induced macrophage inflammation model.The results showed that the yield of polysaccharides from the Castanea mollissima endocarp was 6.73％under the conditions of a liquid-to-solid ratio of 20 mL/g,extraction time of 45 min,and extraction temperature of 60 ℃.The half-maximal inhibitory concentration(IC50)values of the polysaccharides from the Castanea mollissima endocarp for scavenging DPPH and hydroxyl radicals were 0.59 and 0.99 g/L,respectively.The polysaccharides from the Castanea mollissima endocarp could significantly inhibit the release of nitric oxide(NO)in LPS-induced macrophages and reduce the relative expression levels of inflammatory cytokines interleukin-6(IL-6)and interleukin-iβ(IL-1β)mRNA.The study indicated that the polysaccharides from the Castanea mollissima endocarp had good antioxidant function and anti-inflammatory activities and had the potential to be used as functional feed additives.
Keywords：Castanea mollissima endocarppolysaccharidesextraction processantioxidantanti-inflammatory
Publication Date：2025-08-28
Online Publishing Date：2025-10-28(First online date of this platform, not the publication date of the document)
Pages：6( 93-98 )