Differential analysis of ceRNA regulatory network and fat metabolism in adipose tissue of Jilin Hua pigs and Binary pigs
ZHANG Lianjiang
LIU Jianbo
SUN Shuxia
NIU Wei
Abstract：The experiment aimed to investigate both the differences in transcriptional regulation within adipose tissue across pig breeds and the effect of competitive endogenous RNA(ceRNA)networks on adipogenesis.Subcutaneous adipose tissues from six-day-old Jilin Hua pigs and Binary pigs were subjected to transcriptome sequencing(RNA-Seq).The results showed that a total of 1 705 differentially expressed genes(DEGs),130 differentially expressed miRNAs(DE miRNAs),and 129 differentially expressed circRNA(DE circRNAs)were identified.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment pathway analysis revealed that DEGs were significantly enriched in fatty acid metabolism,retinol metabolism,and unsaturated fatty acid biosynthesis pathways centered on fatty acid desaturase 1(FADS1)and 17β-hydroxysteroid dehydrogenase 12(HSD17B12)(P＜0.05).Construction of the ceRNA network showed that sus_circ_0020363,sus_circ_0010212 and sus_circ_0009906 increase the expression levels of FADS1 and HSD17B12 by competitively binding to ssc-novel-mir-48,ssc-mir-338 and ssc-novel-mir-79,thereby relieving the inhibitory effect of miRNA on these two genes.Real-time quantitative PCR(RT-qPCR)validation confirmed significantly higher mRNA expression levels of CCAAT/enhancer-binding protein α(C/EBPα),lipoprotein lipase(LPL),and stearoyl-CoA desaturase(SCD)were significantly higher in Jilin Hua pigs compared to Binary pigs(P＜0.05),consistent with RNA-Seq results.The study indicates that circRNA-miRNA interactions alleviate transcriptional repression of FADS1 and HSD17B12,and forms a synergistic regulatory network with C/EBPα,LPL,SCD and other fat differentiation related genes to promote the generation and storage of fat.It is speculated that this mechanism may be an important molecular basis for the superior quality of local breeds compared with imported breeds.
Keywords：Jilin Hua pigadipose tissuecompetitive endogenous RNAfat metabolism
Publication Date：2025-08-28
Online Publishing Date：2025-10-28(First online date of this platform, not the publication date of the document)
Pages：7( 67-73 )