Fermentation conditions and enzymatic properties of cellulase produced by endophytic Aspergillus niger BGEF25 of Illicium verum
CHEN Xinyi
LI Ziyuan
DONG Baorui
ZHANG Huixiang
JIANG Jicen
LI Haiyun
Abstract：The experiment aimed to study the fermentation conditions and enzymatic properties of cellulase produced by endophytic Aspergillus niger BGEF25 of Illicium verum.Using the single-factor method with carboxymethyl cellulase(CMCase)activity as the indicator,the fermentation conditions for CMCase production by Aspergillus niger BGEF25 were investigated.The results showed that when using 60 g/L rice straw powder as the carbon source,15 g/L soybean powder as the nitrogen source,with an initial pH value of 6.0 for the fermentation medium,a fermentation temperature of 30 ℃,a inoculation rate of 10％,a liquid volume of 100 mL(250 mL test tube),a shaking speed of 140 r/min,and a fermentation time of 96 hours,the activity of the CMCase enzyme was the highest,reaching 205 U/mL.Enzymatic characterization showed that the optimal pH values for CMCase,filter paper enzyme,and β-glucosidase in the cellulase system were 5.0,5.0,and 4.5,respectively,while their optimal temperatures were 45,50,and 50 ℃,respectively.All three enzymes exhibited good stability within pH 4.0～6.0 and at temperatures of 40～60 ℃.Metal salts such as MnCl2 and ZnSO4 can enhance enzyme activity,while HgCl2 and AgNO3 significantly inhibit enzyme activity.The study indicates that the endophytic Aspergillus niger BGEF25 of Illicium verum has excellent cellulase production capacity.
Keywords：cellulaseendophyte of Illicium verumAspergillus nigerenzyme production conditions
Publication Date：2025-08-16
Online Publishing Date：2025-10-14(First online date of this platform, not the publication date of the document)
Pages：6( 91-96 )