Effects of Bacillus amyloliquefaciens GB-9 agent on growth performance and health statu of Boer goats

DAI Binbin; ZHONG Bo; XIE Jieyu; LÜ Zhengbing

doi:10.13557/j.cnki.issn1002-2813.2025.15.004

Abstract：The experiment aimed to investigate the effects of supplementing drinking water with Bacillus amyloliquefaciens GB-9 agent on the growth performance and health status of Boer goats.Active components of the GB-9 agent were identified through PCR amplification and mass spectrometry analysis,and its antimicrobial activity was evaluated via in vitro antibacterial assays.A feeding trial was conducted using Boer goats to screen effective doses of the GB-9 agent.The efficacy of the GB-9 agent in treating acute diarrhea in lambs,as well as its effects on herd immunity and growth performance were evaluated.The results showed that the GB-9 agent contains lipopeptide antimicrobial substances including surfactins,iturins and fengycins,and β-1,3-1,4-glucanase.It exhibited significant inhibitory effects against pathogenic microorganisms such as Staphylococcus aureus and Aspergillus niger,along with potential for modulating gut microbiota.Dose screening identified 0.004％as the effective supplementation concentration.Adding 0.01％GB-9 agent in drinking water cured acute lamb diarrhea and improved gut microbiota abundance,with effects superior to oxy tetracycline.Compared with the control group,the incidences of fever,diarrhea,loss of appetite,urinary tract infection,cold and runny nose,as well as the total incidence rate in the GB-9 bacterial preparation group of Boer goats were significantly decreased(P＜0.05),the final weight and average daily weight gain were significantly increased(P＜0.05).The study indicates that supplementing drinking water with 0.004％Bacillus amyloliquefaciens GB-9 agent enhances growth performance and reduces disease incidence in Boer goats.