Effect of raffinose on proliferation and growth rate of probiotics

BUHELIQIEMU Yushanaji; WANG Jing; ZHANG Jian; YUAN Jiang; LIU Jiancheng; LI Fengming

doi:10.13557/j.cnki.issn1002-2813.2025.14.016

Abstract：The study aimed to systematically evaluate the effect of raffinose as a prebiotic on the proliferation and growth rate of four probiotics through in vitro experiments.Photoelectric turbidimetry was employed to measure its effects on the proliferation of Lactobacillus plantarum,Lactobacillus acidophilus,Bifidobacterium adolescentis,and Bifidobacterium infantis in vitro.Changes in pH and optical density at 625 nm(OD625nm)during growth were monitored to assess its growth rate.The results showed that Bifidobacterium infantis and Bifidobacterium adolescentis reached maximum proliferation at 24 hours of cultivation,while Lactobacillus plantarum and Lactobacillus acidophilus peaked at 48 hours.At 48 hours,with 1.0％D-fructose supplementation,Lactobacillus acidophilus exhibited the highest OD value of 1.681,whereas Lactobacillus plantarum,Bifidobacterium infantis,and Bifidobacterium adolescentis showed OD values of 0.981,0.855,and 0.856,respectively.At 1.0％raffinose supplementation,Lactobacillus acidophilus achieved the highest OD value of 1.906,while Lactobacillus plantarum,Bifidobacterium infantis,and Bifidobacterium adolescentis displayed OD values of 1.050,0.990,and 0.984,respectively.Lactobacillus plantarum reached its maximum growth at 20 hours,Lactobacillus acidophilus at 48 hours,and Bifidobacterium adolescentis and Bifidobacterium infantis at 32 hours.Continuous acid production by all four probiotics led to a sustained decline in pH value during growth.The study indicates that raffinose promotes the in vitro proliferation of these probiotics,with the most significant effect observed on Lactobacillus acidophilus.