Effects of different enrichment methods of Artemia on survival rate,metamorphosis rate,and fatty acid composition of Oratosquilla oratoria larvae

CHEN Chunxiu; MA Chao; YU Yanguang; BO Qikang; SHANG Xiaodi; LU Yunzhao; JIA Lei; WANG Xiaoyu

doi:10.13557/j.cnki.issn1002-2813.2025.14.010

Abstract：The experiment aimed to investigate the effects of differently enriched Artemia on the survival rate,metamorphosis rate,and fatty acid composition of Oratosquilla oratoria larvae.A total of 450 Oratosquilla oratoria larvae were selected and randomly divided into three groups with three replicates per group and 50 larvae per replicate.The unfortified group(group A),emulsified fish oil fortified group(group B),and Schizochytrium sp.fortified group(group C)were fed with Artemia without nutritional fortification as well as those fortified with emulsified fish oil and Schizochytrium sp.powder,respectively.The test period was seven days.The results showed that the survival of Oratosquilla oratoria larvae in group B was significantly higher than in group A(P＜0.05).The metamorphosis rates in groups B and Group C was significantly higher than that in group A(P＜0.05).Groups B and C had lower saturated(SFA)and monounsaturated(MUFA)fatty acid concentrations in Artemia than group A,but higher polyunsaturated(PUFA)content than group A.The content of arachidonic acid(ARA)in each group of halogenated insects was relatively stable,ranging from 1.85％to 1.96％.The group B had the highest content of eicosapentaenoic acid(EPA),accounting for 12.54％,while the group C had the highest content of docosahexaenoic acid(DHA),accounting for 9.36％.In Oratosquilla oratoria larvae,group A had higher MUFA but lower PUFA than groups B and C(P＜0.05).Group B had the highest ARA and EPA concentrations,whereas group C had the highest DHA,both significantly higher than other groups(P＜0.05).In juvenile Oratosquilla oratoria,the concentrations of SFA and MUFA in groups B and C were significantly lower than those in group A(P＜0.05),while the concentrations of PUFA and ARA were significantly higher(P＜0.05).Group B exhibited the highest EPA content,whereas group C showed the highest DHA content,both significantly higher than the other groups(P＜0.05).The study shows that feeding fortified Artemia during the larvae stage of the Oratosquilla oratoria can improve seedling quality,increase PUFA content,and improve survival and metamorphosis rates.Emulsified fish oil has a good effect on increasing the EPA content,while Schizochytrium sp.powder has a good effect on increasing DHA content.