Effects of rumen-protected arginine on rumen fermentation parameters and microbial communities in rumen and intestine of Laoshan dairy goats
WANG Yue
LI Kangxia
JING Maozhan
CHENG Ming
GUO Yixuan
ZHU Fenghua
Abstract：The experiment aimed to investigate the effects of rumen-protected arginine(RP-Arg)on the rumen fermentation parameters and microbial communities in the rumen and intestine of Laoshan dairy goats.A total of twenty 2～3 parity Laoshan dairy goats with similar body weight,milk yield(1.79±0.46)kg/d,and postpartum(63.9±10.2)d were randomly divided into two groups with 10 replicates per group and one goat per replicate.The control group was fed the basal diet,while the experimental group was fed a basal diet supplemented with 50 g/d of RP-Arg per goat.The pre-test period was 15 days,and the formal test period was 60 days.The results showed that the pH value of the rumen fluid in the experimental group significantly decreased(P＜0.05),the concentrations of ammonia nitrogen and total volatile fatty acids increased extremely(P＜0.01),the concentrations of butyric acid and isobutyric acid increased significantly(P＜0.05).The rumen microbial group analysis showed that,the relative abundance of the Patescibacteria phylum was significantly increased in the experimental group(P＜0.05).The relative abundance of the Quinella genus was significantly increased(P＜0.05),and the relative abundance of rumen Bacteroides ruminicola group was significantly decreased(P＜0.05).The intestinal microbial group analysis showed that,the relative abundance of Elusimicrobiota was significantly increased(P＜0.05).The relative abundance of the Ruminococcaceae_unclassified,UCG-009,Anaerovibrio,Elusimicrobium,Eubacterium coprostanoligenes group,Clostridium sensu stricto 6,and Christensenellaceae_unclassified was significantly increased(P＜0.05),the relative abundance of Anaerovibrio and Hydrogenoanaerobacterium was extremely increased(P＜0.01),and the relative abundance of the Bacteroidales_unclassified abundance was extremely decreased(P＜0.01).The study shows that adding 50 g/d of RP-Arg per goat to the diet can optimize the rumen fermentation function of Laoshan dairy goats,promote the proliferation of beneficial microorganisms,improve the utilization efficiency of nutrients,and reduce methane emissions by regulating the hydrogen metabolism pathway.
Keywords：rumen-protected arginineLaoshan dairy goatsrumen fermentation parametersrumen microbiotaintestinal microbiota
Publication Date：2025-07-28
Online Publishing Date：2025-09-18(First online date of this platform, not the publication date of the document)
Pages：7( 10-16 )