Research on preparation of high-soluble protein functional feed by enzyme-microorganism synergistic fermentation of distillers dried grains with solubles(DDGS)
WANG Fuyun
LIU Xiaolan
LI Guanlong
DENG Xiangqing
ZHENG Xiqun
Abstract：The study aimed to enhance the bioavailability of distillers dried grains with solubles(DDGS).Bacillus subtilis,Saccharomyces cerevisiae,and Enterococcus faecalis isolated from naturally fermented apple enzymes,combined with acid protease,are used to prepare DDGS-based fermented feed rich in soluble protein and small peptides.Through primary and secondary screening trials,three dominant fermentation strains are obtained.Orthogonal tests are employed to optimize the seed liquid ratio of these dominant strains.The optimal ratio is determined as Bacillus subtilis CL16∶Saccharomyces cerevisiae BY22∶Enterococcus faecalis JM03=4∶1∶6.Single-factor experiments combined with response surface methodology are used to optimize the fermentation conditions.The optimal conditions are determined as follows:inoculation volume 10％,moisture content 46％,protease dosage 83 U/g,fermentation time 105 hours,and fermentation temperature 28 ℃.Under these optimized conditions,the soluble protein content in the fermented feed reached 134.78 mg/g,and the small peptide content reached 126.35 mg/g.Compared to the pre-fermentation state,the soluble protein content increased by 182％,and the small peptide content increased by 211％.The research demonstrates that microbial-enzymatic synergistic treatment of DDGS can effectively improve its bioavailability and enhance the nutritional value of the feed.
Keywords：distillers dried grains with solublessynergistic action of bacteria and enzymesfermented feedmulti-strain synergy
Publication Date：2025-07-16
Online Publishing Date：2025-09-09(First online date of this platform, not the publication date of the document)
Pages：8( 92-99 )