Effect of drought stress on antioxidant properties of Cyperus esculentus L.

SUN Rui; WANG Rui; GAO Haijuan; WANG Ruoding; LI Wei; ZHANG Jun; WANG Xiaolong; LI Shasha

doi:10.13557/j.cnki.issn1002-2813.2025.13.016

Abstract：The aim of this study was to investigate the effects of drought stress on the antioxidant properties of Cyperus esculentus L.Heiyousha NO.2,Xiaochangli,HB001,NM001,and Zhongyousha NO.1 were selected as the experimental materials.Four treatment levels were set.The CK treatment added distilled water,while the drought stress treatments added 10％,20％and 30％PEG-6000 respectively.After 24 hours of reaction,the indicators such as soluble protein(SP),malondialdehyde(MDA),proline(Pro),superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)of the Cyperus esculentus L.seedlings were determined.The drought resistance ability of each variety of Cyperus esculentus L.was evaluated by principal component analysis.The results showed that the SP content of Heiyousha NO.2 and NM001 gradually increased with the increase of PEG-6000 level,while the MDA content of Heiyousha NO.2,HB001,and Zhongyousha NO.1 showed a change of first decreasing and then increasing with the increase of PEG-6000 level.The Pro content of Heiyousha NO.2 and Zhongyousha NO.1 showed a trend of first decreasing and then increasing with the increase of PEG-6000 level.The CAT and SOD activity of Heiyousha NO.2 and Zhongyousha NO.1 gradually increased with the increase of PEG-6000 level.With the increase of drought stress,the POD activity of five varieties of Cyperus esculentus L.changed in different ways.The comprehensive analysis of the main components revealed that the drought resistance ranking of the five Cyperus esculentus L.varieties was:NM001＞Heiyousha NO.2＞HB001＞Zhongyousha NO.1＞Xiaochangli.The results show that NM001 has the strongest drought resistance,further research can be conducted on the intrinsic connection between drought stress and antioxidant properties of Cyperus esculentus L.