Study on purification,structural characterization,and antioxidant activity of Scenedesmus polysaccharides

WANG Chuanlin; LIU Haowen; LIU Jiaxuan; LIU Pinghuai

doi:10.13557/j.cnki.issn1002-2813.2025.13.014

Abstract：The experiment aimed to explore the purification,structural characterization,and antioxidant activity of polysaccharides from Scenedesmus.Two rounds of column chromatography were conducted to purify the crude polysaccharides from Scenedesmus,and the monosaccharide composition,relative molecular weight,functional groups,thermal stability and microstructure of the polysaccharide components were analyzed.The results showed that four polysaccharide components with relatively high purity were obtained,namely TBP-1,TBP-2,TBP-3 and TBP-4.The main component of TBP-1 was glucose,while the main component of TBP-2 and TBP-3 was galactose,and the main component of TBP-4 was rhamnose.The relative molecular weights of TBP-1,TBP-2,TBP-3 and TBP-4 were 6.45,4.51,5.44 and 9.03 kDa,respectively.Infrared spectroscopy analysis showed that all four polysaccharides contained pyranose ring structures,and TBP-2 and TBP-3 were polysaccharides linked by β-glycosidic bonds.XRD analysis showed that all four polysaccharides had amorphous non-crystalline structures.The triple helix structure analysis showed that TBP-4 had a larger relative molecular weight and a more complex conformation,and had a triple helix structure.Thermal stability analysis showed that TBP-2,TBP-3 and TBP-4 had good thermal stability.All four polysaccharide components exhibited good antioxidant capacity,among which TBP-2 and TBP-3 had the strongest antioxidant activity.The study indicates that Scenedesmus polysaccharides have the potential to be natural antioxidants.