Study on optimizing extraction process of Sargassum weizhouense polysaccharide by response surface methodology and its antioxidant activity
WANG Bixue
ZHANG Ting
TANG Yuhong
DING Yiqu
LU Guangyuan
GAO Yeheng
WANG Qiuhua
WEI Yingyi
Abstract：The experiment aimed to optimize the enzymatic hydrolysis and alcohol precipitation extraction process of Sargassum weizhouense polysaccharides,and to evaluate the in vitro antioxidant activity of Sargassum weizhouense polysaccharides.A single-factor experimental approach was utilized to evaluate the impact of four key parameters,the ratio of liquid to solid,duration of extraction,extraction temperature,and extraction times.Regression model based on response surface methodology(RSM)was developed to forecast the optimal conditions for extraction.The scavenging effect of Sargassum weizhouense polysaccharide on 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and hydroxyl free radicals was determined to explore its neutralization effect on specific free radicals.The results showed that the optimal extraction process obtained by the response surface methodology optimization was a liquid-to-solid ratio of 24 mL/g,a duration of 4 hours for extraction,a temperature of 63 ℃ during extraction,and applying two extraction times.Under this condition,the extraction rate of Sargassum weizhouense polysaccharide could reach 49.52％.When the concentration of Sargassum weizhouense polysaccharide was 2.0 g/L,the maximum scavenging rates of DPPH free radicals and hydroxyl free radicals were 86.46％and 81.84％,respectively.The study indicates that the enzymatic hydrolysis,water extraction and alcohol precipitation extraction process adopted in the experiment yields better results for extracting polysaccharides from Sargassum weizhouense,and the Sargassum weizhouense polysaccharides also exhibit good antioxidant activity.
Keywords：Sargassum weizhouensepolysaccharidesenzymatic hydrolysiswater extraction and alcohol precipitationantioxidant activity
Publication Date：2025-06-28
Online Publishing Date：2025-08-21(First online date of this platform, not the publication date of the document)
Pages：6( 86-91 )