Study on purification process of effective fractions from Gardenia jasminoides based on multi-index component detection and chromatographic technique
LIU Jing
XIA Ziwei
WU Zhigui
Zhong Rui
XU Ruixiang
LIU Yingjiao
FU Xiaomei
Abstract：This study aimed to optimize the extraction and purification processes for total iridoids and total crocins from Gardenia jasminoides.Using geniposide,genipin-l-β-D-gentiobioside,crocin-1,crocin-2,and crocin-3 as evaluation indicators,orthogonal experiments were conducted to investigate extraction time,extraction cycles,and ethanol concentration to optimize the extraction process.Parameters such as macroporous resin type,eluent,elution flow rate,and eluent volume were examined to determine the optimal purification process.The results showed that the optimal extraction conditions were:Reflux extraction with 12 times the volume of 95％ ethanol for 1 hour,followed by filtration and a second reflux extraction with 12 times the volume of 75％ ethanol for 1 hour.The optimal purification process involved using an HP-20 macroporous resin column with an elution flow rate of 4 mL/min.Impurities were removed with 2 BV of water,followed by elution with 4 BV of 25％ ethanol to obtain iridoid component group Ⅰ.Subsequent elution with 3 BV of 40％ ethanol and 4 BV of 95％ ethanol yielded crocin component group Ⅰ.The 40％ ethanol eluate was further purified via a polyamide column:3 BV of 15％ ethanol eluted iridoid component group Ⅱ,while 3 BV of 75％ ethanol eluted crocin component group Ⅱ.Under these conditions,the transfer rates of total iridoids and total crocins were 76.47％ and 70.10％,with dry extract yields of 8.32％ and 3.89％,respectively.This study demonstrates that the developed process is stable,feasible,and achieves high transfer rates of active components,making it suitable for the extraction and purification of effective fractions from Gardenia jasminoides.
Keywords：Gardenia jasminoidesiridoidscrocinmacroporous adsorption resinpolyamidepurification
Publication Date：2025-06-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 85-92 )