Process optimization of complex enzyme-assisted hot water extraction of passion fruit peel polysaccharides and study on their in vitro and in vivo antioxidant activities
WANG Menghui
YI Dandan
ZHAO Linyi
ZHANG Rui
WEI Qianyin
ZHONG Yawen
WU Chunxuan
HE Jiakang
Abstract：This study aimed to optimize the complex enzyme-assisted hot water extraction process for polysaccharides from passion fruit peel and investigate their antioxidant activities through in vitro and in vivo experiments.Single-factor and orthogonal experiments were conducted to screen the optimal ratio of complex enzymes(pectinase,papain,and cellulase).The single-factor test and response surface test were adopted to optimize the process of extracting polysaccharides from passion fruit peels assisted by complex enzyme-assisted hot water,and their antioxidant activities in vivo and in vitro were evaluated.The results showed that the optimal enzyme combination was 0.5％ pectinase,1.5％papain,and 2.0％ cellulase.The best extraction conditions were as follows:Extraction temperature 55 ℃,pH 5.4,and extraction time 149 min,yielding an actual polysaccharide yield of 6.37％.Passion fruit peel polysaccharides exhibited strong scavenging effects on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radicals.The activity of superoxide dismutase(SOD)and the total antioxidant capacity(T-AOC)in the serum of mice in the low-dose group,medium-dose group and high-dose group were significantly higher than those in the blank group(P＜0.05),and the content of malondialdehyde(MDA)in the serum of mice in the medium-dose group and high-dose group was significantly lower than that in the blank group(P＜0.05).This study demonstrates that passion fruit peel polysaccharides possess excellent antioxidant activity,and the findings provide a reference for further development and application of passion fruit peel polysaccharides.
Keywords：passion fruit peelpolysaccharidescomplex enzyme-assisted hot water extractionantioxidant activity
Publication Date：2025-06-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 71-77 )