Evaluation of combined effects of Gentiana macrophylla straw with alfalfa hay,corn straw,and Chenopodium quinoa straw by using in vitro gas production method
HAO Shengyan
PAN Faming
LIU Lishan
LI Chong
TANG Defu
Abstract：This study aimed to evaluate the effects of combinations of Gentiana macrophylla straw with alfalfa hay,corn straw,and Chenopodium quinoa straw on rumen fermentation characteristics in sheep by in vitro gas production method.A ratio of 40∶60 was adopted for the design.For the combinations,the ratios of Gentiana macrophylla straw to alfalfa hay,Gentiana macrophylla straw to corn straw,and Gentiana macrophylla straw to Chenopodium quinoa straw were set at 20∶40,30∶30,and 40∶20 respectively,totaling nine combinations.The results showed that in the Gentiana macrophylla straw and alfalfa hay group the rapid gas production part(a),the slow gas production part(b),the gas production rate constant of b(c),potential gas production(a+b),organic matter degradation rate(OMD),ammonia nitrogen(NH3-N),and the combined effect index of NH3-N in the 20∶40 group were significantly higher than those of in the 40∶20 group(P<0.05).The combined effect index of gas production at 48 h(GP48 h),dry matter degradation rate(DMD),organic matter degradation rate(OMD),and the comprehensive combination effect index(MFAEI)were significantly higher than those of in the 30∶30 and 40∶20 groups(P<0.05).In the Gentiana macrophylla straw and corn straw group,the a+b,the combined effect index of GP48 h,and the combined effect index of NH3-N in the 40∶20 group were significantly higher than those in the 20∶40 group(P<0.05),and the MFAEI was significantly higher than that of the 20∶40 and 30∶30 groups(P<0.05).In the Gentiana macrophylla straw and Chenopodium quinoa straw group,the GP48 h and the combined effect index of NH3-N in the 20∶40 group were significantly higher than those in the 40∶20 group(P<0.05),and the combined effect index of GP48 h and MFAEI were significantly higher than those in the 30∶30 and 40∶20 groups(P<0.05).The study shows that Gentiana macrophylla straw and alfalfa hay exhibited the optimal synergistic effect at a ratio of 20∶40,while the optimal mixing ratios for Gentiana macrophylla straw with corn straw and Chenopodium quinoa straw were 40∶20 and 20∶40,respectively.
Keywords：Gentiana macrophylla strawin vitro gas productionfermentation propertiescombination effects
Publication Date：2025-05-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 115-119 )