Cloning,bioinformatics and tissue expression analysis of STIMATE gene in Xuefeng black-bone chicken
YU Yan
CHANG Xiaopeng
Abstract：The aim of this study was to clone the STIM-activating enhancer(STIMATE)gene of Xuefeng black-bone chickens and analyze its bioinformatics and tissue expression.The cDNA of tissue was used as template to clone the CDS region of STIMATE gene and sequence it which was analyzed by online software for bioinformatics analysis.qPCR technology was used to detect the expression level of STIMATE gene in nine tissues of heart,liver,lung,spleen,kidney,proventriculus,gizzard,breast muscle and thigh muscle.The results showed that the CDS length of the STIMATE gene was 897 bp,with one synonymous mutation encoding 298 amino acids,and its amino acid sequence was 100%similar to that of Gallus gallus.STIMATE was expressed in various tissues of Xuefeng black-bone chicken,among which the expression level was the highest in the lungs and the lowest in the pectoral muscles.The study indicates that the experimental results can provide a reference for further exploring the function of the STIMATE gene in chickens.
Keywords：Xuefeng black-bone chickenSTIMATE genegene clonebioinformatics
Publication Date：2025-05-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 62-67 )