Process optimization of macroporous resin purification of lignans from Fructus arctii and its antipyretic and analgesic effects
YAN Guoqing
ZENG Xueyan
YIN Huihui
HUANG Yunfu
JIANG Yuanming
LAN Tianyan
HE Shenhong
LIU Wei
Abstract：This study aimed to investigate the purification process of lignans from Fructus arctii using macroporous resin and evaluate their antipyretic and analgesic effects.An ultra-performance liquid chromatography(UPLC)method was established to determine the concentrations of arctiin and arctigenin.The purification process for arctiin and arctigenin using macroporous resin was optimized.Additionally,dry yeast-induced fever and acetic acid-induced pain mouse models were established to explore the antipyretic and analgesic effects of Fructus arctii lignans.The results showed that the optimal purification conditions were as follows:AB-8 resin,sample loading volume of 1 BV,sample loading flow rate of 2 BV/h,elution with 4 BV of pure water followed by 5 BV of 50%ethanol,and collection of the 50%ethanol eluate.After purification,the purities of arctiin and arctigenin reached 68.52%and 8.07%,with transfer rates of 81.07%and 73.47%,respectively.In the antipyretic test,compared with the model group,the high-dose purified group showed significantly reduced body temperature changes at 6 and 7 h(P<0.05),while the medium-dose purified group exhibited significantly reduced changes at 7 h(P<0.05).At 6 h,the medium-and low-dose purified groups showed extremely reductions in body temperature changes(P<0.01).Compared with the model group,all treatment groups exhibited extremely decreases in serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and hypothalamic prostaglandin E2(PGE2)levels(P<0.01).The low-dose and medium-dose purified groups showed extremely reductions in hypothalamic cyclic adenosine monophosphate(cAMP)content(P<0.01),while the high-dose group showed a significant reduction(P<0.05).In the analgesic test,the pain inhibition rates were 36.59%for the crude extract group and 57.01%,50.65%,and 62.56%for the low-dose,medium-dose,and high-dose purified groups,respectively.The study demonstrates that the purified lignans from Fructus arctii possess antipyretic and analgesic effects,with the antipyretic mechanism likely related to pyrogenic factors and hypothalamic thermoregulatory mediators.
Keywords：Fructus arctiilignansmacroporous resinantipyreticanalgesic
Publication Date：2025-04-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 98-104 )