Effects of Allium mongolicum Regel polysaccharide on antioxidant function and its mechanism in diquat-challenged mice
NAN Guorong
ZHENG Yankai
CHEN Yu
LI Dabiao
XING Yuanyuan
Abstract：This study aimed to investigate the alleviating effects of Allium mongolicum Regel polysaccharide on oxidative stress in mice and its molecular mechanisms.Thirty-six SPF-grade mice were randomly divided into three groups:Control group(CON group),diquat-challenged group(DQ group),and AMRP+Diquat-challenged group(AMRP+DQ group),with 12 replicates per group,one mouse per replicate.AMRP+DQ group was orally administered 0.2 mL of 80 g/L Allium mongolicum Regel polysaccharide solution daily,while CON and DQ groups received the same volume of saline.After the experiment,fast for 12 hours,mice in DQ and AMRP+DQ groups were subcutaneously injected with 10 mg/kg BW diquat solution.The pre-trial period lasted 7 days,followed by a 21-day formal trial.The results showed that compared with DQ group,the average daily gain in AMRP+DQ group was significantly increased(P<0.05).Compared with DQ group,the content of 8-hydroxydeoxyguanosine(8-OHDG),malondialdehyde(MDA)and protein carbonyl(PCO)in serum of AMRP+DQ group was significantly decreased(P<0.05),and the total antioxidant capacity(T-AOC)was significantly increased(P<0.05).The content of 8-OHDG and MDA in small intestine were extremely decreased(P<0.01),PCO content was significantly decreased(P<0.05),total antioxidant capacity(T-AOC)and total superoxide dismutase(T-SOD)activity were extremely increased(P<0.01),and catalase(CAT)activity was significantly increased(P<0.05).Compared with DQ group,mRNA expression levels of glutathione catalase(GSH-Px),CAT,superoxide dismutase(SOD),heme oxygenase-1(HO-1)and nuclear factor E2-related factor 2(Nrf2)in AMRP+DQ group were significantly increased(P<0.05).The mRNA expression of Kelch-like ECH associated protein 1(Keap1)was significantly decreased(P<0.05).The study indicates that dietary supplementation with Allium mongolicum Regel polysaccharide enhances growth performance,improves serum and intestinal T-AOC and activities of CAT,GSH-Px,and T-SOD,reduces oxidative markers(8-OHDG,MDA,PCO),and alleviates oxidative stress in mice by modulating the Keap1/Nrf2/HO-1 signaling pathway.
Keywords：Allium mongolicum Regel polysaccharidediquatoxidative stressKeap1/Nrf2/HO-1 signaling pathway
Publication Date：2025-04-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 67-72 )