Optimization of polyphenol extraction from eucalyptus leaves with deep eutectic solvents and study on its antioxidant activity
JIANG Lirong
ZENG Xiangchu
ZHANG Gaorong
WANG Yanping
LI Jinyue
CHEN Juanlin
HUANG Xiuxiang
Abstract：This study optimized the extraction process of polyphenols from eucalyptus leaves using deep eutectic solvents with choline chloride and betaine as hydrogen bond acceptors through single-factor and orthogonal experiments,and evaluated their antioxidant activity.The results showed that the optimal conditions were as follows:A betaine-to-ethylene glycol ratio of 2∶1,water content of 50%,liquid-to-material ratio of 40 mL/g,and ultrasonic power of 300 W,ultrasonic temperature of 75℃,ultrasonic time of 50 min.Under these conditions,the extraction yield of eucalyptus polyphenols reached 22.39%,significantly higher than that of traditional solvents.The eucalyptus leaf extract exhibited stronger scavenging effects on 2,2-diazo-di(3-ethyl-benzothiazolin-6-sulfonic acid)diamiammonium salt(ABTS+)radicals compared to 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and nitrogen dioxide radicals,with maximum scavenging rates of 98.8%,83.5%,and 77.5%,respectively.The corresponding IC50 values were 4.41,4.82,and 324.70 mg/L.The study demonstrates that the betaine/ethylene glycol-based deep eutectic solvents system achieves high extraction efficiency and excellent antioxidant activity for eucalyptus polyphenols,providing a reference for the future development and utilization of eucalyptus resources.
Keywords：deep eutectic solventseucalyptus leavespolyphenolsantioxidant activityprocess optimization
Publication Date：2025-04-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 96-101 )