Studies on effect of fisetin in mitigating aflatoxin toxicity in mice
WANG Mengyin
LIU Hao
LI Kun
HUANG Youqian
YANG Daize
LIU Kun
PENG Tao
QIN Shunyi
Abstract：The study aims to explore the effect of fisetin on mitigating aflatoxin toxicity in mice.Sixty 4-week-old Kunming mice were selected,randomly divided into three groups,each with four replicates and five mice(equal sex)per replicate.The control group was fed a basic diet.The AF group received 250 μg/kg of aflatoxin toxicity in the diet.The FI group received 250 μg/kg of aflatoxin toxicity and 50 mg/kg of fisetin.The pre-trial period one weeks,and the formal trial lasted five weeks.The results showed that compared to the control group,the AF group had significantly lower final weight,weight gain,and feed intake(P<0.05),and a significantly higher feed-to-weight ratio(P<0.05).Compared to the AF group,the FI group had significantly higher final weight,weight gain,and feed intake(P<0.05),and a significantly lower feed-to-weight ratio(P<0.05).Compared to the control group,the AF group had significantly higher alanine aminotransferase(ALT)activity(P<0.05)and extremely higher aspartate aminotransferase(AST)activity(P<0.01).Compared to the AF group,the FI group had significantly lower ALT activity(P<0.05)and extremely lower AST activity(P<0.01).Compared to the control group,the AF group had significantly lower catalase(CAT),glutathione peroxidase(GSH-Px)activities and total antioxidant capacity(T-AOC)(P<0.05),and significantly higher malondialdehyde(MDA)content(P<0.05).Compared to the AF group,the FI group had significantly higher GSH-Px activity and T-AOC(P<0.05),and significantly lower MDA content(P<0.05).Compared to the control group,the AF group had significantly higher interleukin-6(IL-6)and interleukin-1β(IL-1β)concentrations(P<0.05),and significantly lower interleukin-4(IL-4),immunoglobulin G(IgG),and immunoglobulin A(IgA)concentrations(P<0.05).Compared to the AF group,the FI group had significantly lower IL-6 and IL-1β concentrations(P<0.05),and significantly higher IL-4 and IgG concentrations(P<0.05).The study shows that fisetin can mitigate aflatoxin toxicity in mice by improving growth performance,biochemical indicators,antioxidant function and regulating immunity.
Keywords：fisetinaflatoxin toxicitygrowth performancebiochemical indicatorsantioxidantimmunitymice
Publication Date：2025-04-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 91-95 )