Study on production of δ-aminolevulinic acid by fermentation of complex yeast
LIN Ziying
REN Wenbin
XIN Xuan
YANG Xiaohui
WANG Wei
Abstract：The research aims to improve the production of δ-aminolevulinic acid(δ-ALA)in yeast culture by compounding strains and optimizing cultivation conditions.The experiment selected a compound of Candida lipolytica and Saccharomyces cerevisiae FQ17,and optimized the culture medium and conditions through single factor and orthogonal experiments.The production of δ-ALA in yeast culture was determined by spectrophotometry.The results showed that according to single factor and orthogonal experiment,the optimal culture conditions were as follows:Strain ratio 1∶2,inoculation amount 8%,molasses amount 4.75%,urea amount 0.050%,glucose amount 2.25%,glycine amount 20%,zinc sulfate amount 0.055%,magnesium sulfate amount 0.100%,iron sulfate amount 0.055%and chlorine sodium content 0.005%,the yield of δ-ALA in the yeast culture could reach 471.40 mg/kg.The research results indicate that composite yeast can significantly increase the production of δ-ALA in yeast culture.
Keywords：yeast cultureδ-aminolevulinic acidcompounding strainscultivation condition
Publication Date：2025-04-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 73-78 )