Study on diversity and functional prediction of Cervus nippon rumen microbiota based on high-throughput sequencing of 16S rRNA
QU Lei
GUAN Shiyu
GU Xingliang
QIN Lihong
Abstract：The experiment was to investigate the microbial diversity and function in the rumen of adult female Cervus nippon,analyzed the gastric flora structure and predicted the functions of Cervus nippon tumor samples using 16S rRNA high-throughput sequencing technology and PICRUSt2.The results showed that a total of 289 424 high-quality sequences were obtained by Illumina NovaSeq sequencing platform,with clustering analysis identifying a total of 7 547 operational taxonomic units(OTUs).The alpha diversity indices indicated that the rumen microbial community in Cervus nippon had high richness(Chao1=1 510.99,Ace=1 520.92)and diversity(Simpson=0.99,Shannon=8.50).The dominant phyla were Firmicutes(51.47%)and Bacteroidota(36.94%).The dominant bacterial orders were Bacteroidales(36.91%),Lachnospirales(15.32%),and Oscillospirales(10.54%).The dominant genera were Prevotella(10.78%),uncultured_rumen_bacterium(9.27%),Rikenellaceae_RC9_gut_group(8.66%).KEGG database analyzed six primary level metabolic pathways,COG database distributed in 24 COG categories.The functional categories mainly focus on general functional prediction,amino acid transport and metabolism,translation,ribosome structure and biotransformation,transcription,transport and metabolism of carbohydrates.The study indicates that the rumen colonies of Cervus nippon has obvious diversity and contain abundant cellulose-degrading microorganisms.The test results can provide a reference for the exploration of Cervus nippon rumen microbial functional genes.
Keywords：Cervus nipponrumen microorganisms16S rRNAfunctional prediction
Publication Date：2025-03-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 102-106 )