Effects of compound probiotic preparation on growth performance,intestinal microbiota,and serum hormones levels in lambs

WEI Congcong; ZHOU Qing; ZHANG Guangping

doi:10.13557/j.cnki.issn1002-2813.2025.05.004

Abstract：This study investigated the effects of different levels of compound probiotic preparation on growth performance,intestinal microbiota,and serum hormone levels in lambs.Forty-eight two-month-old Hu lambs were randomly divided into four groups with four replicates per group and three lambs per replicate.The experimental diets were supplemented with 0(control group,CK),0.5%(group A),1.0%(group B),and 2.0%(group C)compound probiotic preparation.The pre-test lasted for seven days,and the formal test lasted for 60 days.The results showed that compared with the CK group,the average daily gain of group B lambs significantly increased(P<0.05),and the feed to gain ratio significantly decreased(P<0.05).Compared with CK group,the Chao index and the relative abundance of Firmicutes in intestinal tract of lambs in group B were significantly increased(P<0.05),and the relative abundance of Bacteroides and Proteobacteria in groups B and C were significantly decreased(P<0.05).The relative abundance of Ruminococcus in all experimental groups significantly increased(P<0.05),whereas Succinivibrio decreased(P<0.05).The relative abundance of Prevotella and Bacteroides in group B were significantly increased(P<0.05).Christensenellaceae and Lachnospiraceae significantly increased in group B(P<0.05),and Escherichia in groups B and C was significantly decreased(P<0.05).Compared with CK group,the serum cortisol(COR)levels in groups B and C was significantly decreased(P<0.05),epinephrine(EPI)level in group B and lipopolysaccharide(LPS)level in groups A and B were significantly decreased(P<0.05).The study indicates that dietary supplementation of 1.0%compound probiotic preparation has a good effect on improving the growth performance,intestinal health and body health of lambs.