Study on response surface optimization of ultrasonic-assisted extraction of total flavonoids from Epimedium and its antioxidant activity
WANG Lingzhi
CHEN Ping
GUO Zhenhua
ZHANG Honglei
Abstract：The experiment was aimed to study the extraction method of total flavonoids from Epimedium and its antioxidant activity.Based on the results of single factor experiment,response surface analysis was used to optimize the extraction conditions of total flavonoids of Epimedium,and its antioxidant activity was systematically evaluated.The results showed that the optimal extraction parameters were ethanol concentration,liquid-solid ratio,extraction temperature and extraction time of 70％,25 mL/g,70 ℃ and 60 min,respectively.Under the optimal conditions,the extraction rate of total flavonoids of Epimedium was 7.65％,which was very close to the predicted value of 7.69％.The IC50 value of the extract of total flavonoids from Epimedium for scavenging 2,2-diphenyl-1-picrohydrazyl(DPPH)free radicals was 82.065 mg/L,and the IC50 value for scavenging 2,2'-diazo-bis(3-ethylbenzothiazolin-6-sulfonic acid)(ABTS+)free radicals was 78.259 mg/L.The study indicates that the optimization of the ultrasound-assisted extraction process by response surface methodology is feasible,and total flavonoids from Epimedium had strong antioxidant activity and the potential to be used as an antioxidant feed additive.
Keywords：Epimediumultrasound-assisted extractionresponse surface methodologyantioxidant activity
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 92-97 )