Optimization of hydrolysis process of konjac fly powder with different proteases
FU Jiarong
MA Shangxuan
HUANG Kechang
LAN Xiuhua
XU Wenting
YANG Yuexue
WEI Yuanmiao
GUO Gangjun
Abstract：The study aimed to investigate the enzymolysis effects of different proteases on konjac fly powder.The liquid method was used to enzymolysis konjac fly powder.Single factor and orthogonal tests were used to optimize the enzymolysis process of different proteases(papain,neutral,acid,alkaline,and bromelain)based on the polypeptide content in the enzymolysis solution.The polypeptide contents of different proteases in the enzymolysis solution were compared under the optimal enzymolysis conditions.The results showed that the optimum conditions and enzymolysis effects of different proteases were different,and the enzymolysis impact could be improved significantly by optimizing the enzymolysis process.The enzymolysis effect of acid protease on konjac fly powder was better than that of the other four proteases.The optimal enzymolysis conditions of acid protease were enzymolysis temperature of 65 ℃,pH value of 1.5,enzymolysis time of 3.0 h,enzymolysis amount of 3 600 U/g,and the polypeptide content of the enzymolysis solution was 7.51 g/L.The study indicates that enzymatic hydrolysis using acid protease can facilitate the high-value utilization of konjac powder in the feed industry.
Keywords：konjac fly powderproteaseenzymatic hydrolysateprocess optimizationpolypeptide content
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 74-79 )