Regulatory effect of FEA on histone acetylation modification in immune cells induced by PRV infection in vivo and in vitro
YU Xiaoli
YUAN Haifeng
HUANG Changqiao
JIANG Liqun
LI Yanhua
HU Xiaodong
REN Chunzhi
HU Tingjun
Abstract：The study aimed to observe the regulatory effect of the ethyl acetate fraction of Fagopyrum flavonoids(FEA)on histone acetylation modification in mice and cells infected with pseudorabies virus(PRV).The experiment consisted of in vivo and in vitro parts.In the in vitro experiment,there were cell control group,trichostatin A(TSA)group,PRV group,rutin group,PRV+FEA 25 group,PRV+FEA 50 group,and PRV+FEA 100 group.In the in vivo experiment,there were blank control group,TSA group,PRV group,rutin group,PRV+FEA 50 group,PRV+FEA 100 group,and PRV+FEA 200 group.The enzyme activities of histone acetyltransferase 1(HAT1)and histone deacetylase 1(HDAC1),the mRNA expression levels of HAT1 and HDAC1,and the acetylation levels of histone H3(AcH3)and H4(AcH4)were measured.The results showed that compared with the PRV group,the activity of HAT1 in RAW264.7 cells in the FEA groups was extremaly decreased(P＜0.01),and the activity of HDAC1 in the PRV+FEA 25 group and PRV+FEA 50 group was significantly increased(P＜0.05).The mRNA expression level of HAT1 in the PRV+FEA 50 group and PRV+FEA 100 group was extremely decreased(P＜0.01),while the mRNA expression level of HDAC1 in the PRV+FEA 25 group and PRV+FEA 50 group was significantly increased(P＜0.05),The acetylation level of AcH3 in the PRV+FEA 25 group and PRV+FEA 100 group,and the acetylation level of AcH4 in the PRV+FEA 50 group and PRV+FEA 100 group were extremely decreased(P＜0.01),and the acetylation level of AcH3 in the PRV+FEA 50 group was significantly decreased(P＜0.05).Compared with the PRV group,the activity of HAT1 in the splenocytes of mice in the PRV+FEA 100 group and PRV+FEA 200 group was extremely decreased(P＜0.01),while the activity of HDAC1 was extremely increased(P＜0.01).The mRNA expression level of HAT1 in the spleen of mice in the PRV+FEA 100 group and PRV+FEA 200 group was extremely decreased(P＜0.01),the mRNA expression level of HDAC1 in the spleen of mice in the PRV+FEA 50 group was significantly increased(P＜0.05),and the mRNA expression level of HDAC1 in the spleen of mice in the PRV+FEA 100 group and PRV+FEA 200 group was extremely increased(P＜0.01).The acetylation expression level of AcH3 in the spleen of mice in the PRV+FEA 50 group and PRV+FEA 100 group,and the acetylation expression level of AcH4 in the spleen of mice in the PRV+FEA 100 group and PRV+FEA 200 group were extremely decreased(P＜0.01),and the acetylation expression level of AcH3 in the spleen of mice in the PRV+FEA 200 group was significantly decreased(P＜0.05).The study showed that FEA could regulate the level of histone acetylation modification in immune cells induced by PRV infection in vivo and in vitro.
Keywords：ethyl acetate fraction of Fagopyrum flavonoidspseudorabies virushistone acetylation
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 56-61 )