Fermentation quality of natural stylo silage and isolation and identification of Clostridium from silage
WANG Jian
HUANG Naixin
WANG Musen
FU Lingling
LI Xuefeng
Abstract：The study aimed to investigate the fermentation quality of natural stylo silage and isolation and identification of Clostridium from silage.Ensiling stylo 60 days was selected as research material,and its fermentation quality and nutrient composition were determined,and using conventional cultivation methods and 16S rDNA sequence analysis to identify isolated Clostridium in silage.The results showed that the vegetative stage of natural stylo silage pH value was 5.31,ammonia nitrogen/total nitrogen was 39.39％and butyric acid content was 0.77％of dry matter.A strain of Clostridium butyricum was isolated from stylo silage,which was identified by morphological observation,physiological and biochemical tests,molecular biological identification and named Clostridium butyicum 4233.The minimum optical density values(OD600nm)of strain Clostridium butyricum 4233 were 0.026 and 0.223 at 15℃ and pH 3.5,respectively.The OD600nm values were higher than 1.600 at 35～45℃,and the OD600nm values were about 1.600 at pH value 7.5～9.0.The study indicates that the natural silage of stylo was a typical clostridial fermentation,and clostridial species in the silage was Clostridium butyricum.
Keywords：stylotropical grassfermentation qualityClostridium butyricumisolation and identification
Publication Date：2025-02-16
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 103-107 )