Effects of replacing fishmeal with plant protein and adding lentinacin on growth performance,liver antioxidant capacity,and liver metabolites of Micropterus salmoides

LI Sijie; SUN Zhenzhu; YU Zhoulin; LIU Guangye; ZHOU Guoyong; WANG Hanhua; ZHOU Meng; TAN Xiaohong

doi:10.13557/j.cnki.issn1002-2813.2025.03.012

Abstract：The experiment aimed to investigate the effects of replacing fishmeal with plant protein and adding lentinacin on the growth performance,liver antioxidant capacity,and liver metabolites of Micropterus salmoides.A total of 360 juvenile Micropterus salmoides with an initial body weight of(28.83±0.01)g were randomly divided into three groups,each group with four replicates,and 30 fish per replicate.The control group(CK group)was fed a basal diet,the plant protein replaced fish meal group(PP group)was replaced by 30％fish meal with plant protein,and fish meal with plant protein and added with lentinin group(MR Group)was added with 30 g/kg lentinacin on the basis of PP group.The experimental period lasted for 56 days.The results showed that the addition of lentinacin in the plant protein replacement diet had no significant effect on the growth performance of Micropterus salmoides(P＞0.05).The total antioxidant capacity(T-AOC)of the MR group was significantly higher than that of the PP and CK groups(P＜0.05),and the malondialdehyde(MDA)content in the MR group was significantly lower than that in the PP group(P＜0.05).A total of 10 upregulated metabolites were identified between the CK and PP groups,mainly including lysophosphatidylcholine,hydroxy fatty acid branched fatty acid esters,phosphatidylcholine,and soy saponin Ⅰ,which were involved in four metabolic pathways such as linoleic acid metabolism.Between the PP and MR groups,four upregulated and 13 downregulated metabolites were identified,mainly including L-glutamate,lysophosphatidylcholine,phosphatidylcholine,UDP-galactose,and phosphatidylethanolamine,which were involved in 24 pathways related to carbohydrate,lipid,and nucleic acid metabolism.The study indicates that adding lentinacin to the plant protein replacement diet mitigates the negative effects of plant protein on the liver of Micropterus salmoides.It reduces lipid peroxidation in the liver,enhances the antioxidant capacity,and alleviates the expression of lipid metabolites in the liver of Micropterus salmoides.This effectively improves the liver tissue damage in Micropterus salmoides and protects the integrity of liver tissue.