Identification of myogenic regulatory factor family genes and effects of culture mode on its expression,growth and muscle texture in Cyprinus carpio var.Yibu
LI Xiaodong
JIAO Fei
LI Lin
HU Jianyong
GAO Pan
WANG Xue
LIU Jing
SHI Chunming
Abstract：The aim of this experiment was to explore the myogenic regulatory factor family genes in easy-catching carp,and the effects of culture mode on gene expression,growth and muscle texture of Cyprinus carpio var.Yibu.A total of 90 Cyprinus carpio var.Yibu of uniform size were selected and randomly divided into two groups,with three replicates per group and 15 carps per replicate.Both the still water group(CK group)and the flowing water group(EG group)were fed with carp fingerling compound feed.The pre-test period was one week,and the formal test period was 73 days.The results showed that through NCBI BLAST comparison,the myogenic factor(myf6)and myogenic differentiation factor(myoD)of Cyprinus carpio var.Yibu were highly homologous with those of common carp,while myogenin(myoG)was evolutionarily distinctly branched.Usingβ-actin as the reference gene,quantitative real-time PCR revealed that under still water breeding conditions,the expression levels of myf6 and myoD in Cyprinus carpio var.Yibu were significantly higher than those in the flowing water group(P＜0.05),under both breeding modes,the expression of the family member myoG showed no significant difference(P＞0.05).There were no significant differences in growth performance,muscle texture,and nutritional characteristics between the two breeding modes(P＞0.05).The study indicates that due to the high cost of intensive breeding,the selection of Cyprinus carpio var.Yibu as an object for intensive recirculating aquaculture should be considered carefully.
Keywords：Cyprinus carpio var.Yibumyogenic regulatory factorgrowthmuscle qualitygene expression
Publication Date：2025-01-14
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 90-94 )