Effects of chromium methionine on growth performance,antioxidant,serum biochemical indicators,and hepatopancreas health of common carp fed with high carbohydrate diet

CUI Pei; LI Nan; LI Min; BO Limei; GENG Zhenzhou; YANG Jun; ZHANG Yongming; SUN Jinhui

doi:10.13557/j.cnki.issn1002-2813.2025.01.015

Abstract：The experiment aimed to investigate the effects of adding chromium methionine(CrMet)to high carbohydrate feed on the growth performance,antioxidant capacity,serum biochemical indicators,and hepatopancreas health in common carp.A total of 180 healthy carp with an initial weight of(86.61±6.68)g were randomly divided into three groups,with three replicates per group and 20 carp per replicate.Three types of feed were prepared:Control group(CK,carbohydrate level 25％),high carbohydrate group(HCD,carbohydrate level 45％),and high carbohydrate feed supplemented with CrMet(HCD+Cr,1 mg/kg Cr3+).The preliminary period was one week,and the formal experimental period was 60 days.The results showed that long-term feeding with high carbohydrate feed did not negatively affect the growth performance of common carp,but it led to a significant increase in the levels of glucose(GLU),low-density lipoprotein(LDL),glutamic-pyruvic transaminase(ALT),alkaline phosphatase(AKP)activities,and malondialdehyde(MDA)content in the serum and hepatopancreas of common carp(P＜0.05),and a significant decrease in total antioxidant capacity(T-AOC),superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities(P＜0.05),causing oxidative stress.Prolonged oxidative stress conditions significantly increased the levels of pro-inflammatory factors interferon-γ(IFN-γ)and interleukin-1β(IL-1β)in common carp(P＜0.05).The addition of CrMet had no significant effect on the growth performance of common carp(P＞0.05),but compared with the HCD group,the levels of GLU,LDL,GPT,and aspartate aminotransferase(AST)activity,AKP activity,MDA content,and IL-1β,IFN-γ,and tumor necrosis factor-α(TNF-α)levels in the serum of common carp were significantly reduced(P＜0.05),while the levels of high-density lipoprotein(HDL),GSH-Px,SOD,and CAT activities were significantly increased(P＜0.05).Histological results showed that the addition of CrMet could significantly improve the degree of degeneration of common carp hepatocytes.The study indicates that after consuming high carbohydrate feed,common carp were in a state of oxidative stress,inducing inflammatory responses.The addition of 1 mg/kg CrMet can enhance the antioxidant capacity of common carp hepatopancreas,alleviate inflammation induced by high carbohydrate feed,and mitigate liver damage.