Effect of adding brown algae extracts to feed on amino acid of muscle in Micropterus salmoides

SHEN Jiajia; WEI Yunyong; LU Bo; WANG Qiaozhen; LIU Hongxiang; WANG Mengran; HUANG Shushi; MA Yanqun

doi:10.13557/j.cnki.issn1002-2813.2025.01.014

Abstract：The experiment aimed to investigate the effects of adding brown algae extracts to feed on the amino acid composition of muscle in Micropterus salmoides.A total of 630 Micropterus salmoides with good condition and similar body weight(33.33±1.95)g were selected and randomly divided into six groups,with three replicates per group and 35 Micropterus salmoides per replicate.The control group(Group A)was fed a basal diet,while Groups B,C,D,E,and F were fed diets in which wheat flour was replaced with 0.1％sodium alginate,0.1％oligosaccharide Ⅰ,0.1％oligosaccharide Ⅱ,0.2％brown algae powder,and 0.2％brown algae powder hydrolysate,respectively.The test period was 56 days.The results showed that compared to the control group,the crude fat content in the muscle of largemouth bass in Groups C,D,and E was significantly reduced(P＜0.05).Compared to the control group,the methionine(Met)content in all experimental groups was significantly increased(P＜0.05),while the lysine(Lys),isoleucine(Ile),valine(Val),threonine(Thr),leucine(Leu),aspartic acid(Asp),glutamic acid(Glu),and tyrosine(Tyr)contents in Group B were significantly decreased(P＜0.05).The first limiting amino acid in the muscle of largemouth bass in all groups was methionine+cysteine(Met+Cys),and the second limiting amino acid was valine(Val).The essential amino acid index was in the order of Group E＞Group D＞Group F＞Group C＞Group B＞Group A,with F values ranging from 2.34 to 2.44.Amino acid scoring and chemical scoring showed that compared to the control group,the content of methionine+cysteine(Met+Cys)in all Groups was significantly increased(P＜0.05).The study indicated that brown alga extract does not have a negative impact on the conventional nutritional components of Micropterus salmoides muscle.Several brown alga extracts can increase the Met+Cys content in the muscle of Micropterus salmoides.Adding 0.1％sodium alginate to the feed leads to a decrease in the total amino acid content in the muscle of Micropterus salmoides.Adding 0.2％brown alga powder hydrolysate is somewhat beneficial for improving the flavor of Micropterus salmoides muscle.Brown alga extract as a feed additive for Micropterus salmoides can improve the amino acid composition and muscle nutritional value of Micropterus salmoides.