Effects of guanidinoaceticacid,bacterial enzyme complex on serum biochemical indexes,semen quality,and antioxidant indexes of ram
LI Xiuli
Abstract：The experiment aimed to investigate the effects of adding guanidine acetic acid(GAA),s.cerevisiae and cellulase complex(SCC)to the diet on the serum biochemical indexes,semen quality,and antioxidant indexes of rams.A total of 24 healthy Dorper rams with a body weight of(75.0±2.5)kg were randomly divided into six groups,with four replicates per group and one ram per replicate.Using a 3 × 2 factorial completely randomized trial design was employed,with GAA(0,0.03％,0.06％)× SCC(0,0.01％)to form six groups,namely(G0,G1,G2,G3,G4,and G5 groups).The preliminary period lasted seven days,and the formal period lasted 60 days.The results showed that the 0.06％GAA group significantly increased the total protein(TP),insulin(INS),growth hormone(GH),testosterone(TE)content,and aspartate aminotransferase(AST)activity in the serum(P＜0.05),and significantly decreased glucose(GLU)content(P＜0.05).The 0.01％SCC group significantly increased TP content(P＜0.05),the GAA × SCC had significant interactive effects on TP,albumin(ALB),GH,and TE(P＜0.05).The 0.06％GAA group and the 0.01％SCC group significantly improved ram sperm vitality and membrane integrity(P＜0.05),and significantly reduced sperm deformity rate(P＜0.05),the GAA × SCC had significant interactive effects on semen quality indicators(P＜0.05).The 0.06％GAA group significantly increased the activity of superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)in the seminal plasma and serum(P＜0.05),the 0.01％SCC group significantly increased serum SOD activity,significantly increased seminal plasma and serum T-AOC(P＜0.05),and significantly decreased malondialdehyde(MDA)content in seminal plasma and serum(P＜0.05),the GAA × SCC had significant interactive effects on SOD and glutathione peroxidase(GSH-Px)activity,MDA content,and T-AOC(P＜0.05).The study indicates that the simultaneous addition of 0.06％GAA and 0.01％SCC in the diet can effectively improve the ejaculate volume,semen quality,testosterone content,and protein content,enhance the antioxidant capacity in seminal plasma and serum of rams.
Keywords：guanidine acetic acids.cerevisiae and cellulase complexramssemen qualityantioxidant indexes
Publication Date：2025-01-14
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 14-20 )