Study on regulatory mechanism of green-shelled eggshell color differences in Xuefeng black-boned chickens based on metabolomics
GUO Xin-yi
YAO Ya-ling
LI Mei-chun
ZI Qiong-tao
ZHOU Yu-xuan
HUANG Jiang-ze
GUO Song-chang
Abstract：The experiment aimed to investigate the key metabolites and metabolic pathways affecting the depth of green color in green eggshells of Xuefeng black-boned chicken.The experiment involved continuously recording the color hue values(L*and a*)of eggs laid by 100 chickens over a one-month period.Based on the a*values,three chickens with light green eggshells(QL group)and three with dark green eggshells(SL group)were selected to collect eggshell gland samples for metabolomic analysis.The results showed that compared to the QL group,the SL group had 10 upregulated and 4 downregulated metabolites,which mainly included pyroglutamic acid,adipic acid,uric acid,ellagic acid,D-ribulose 5-phosphate,guanine nucleoside,hexadecanedioic acid,uracil,leucine phosphatidylcholine,vinyl phosphatidylcholine,choline,purine,and lysophosphatidylethanolamine,among others.Ten pathways were enriched,including cpurine metabolism,glutathione metabolism,and riboflavin metabolism.The study suggests that changes in eggshell color may be influenced by adipic acid,D-ribulose 5-phosphate,leucine phosphatidylcholine,lysophosphatidylethanolamine,and the pentose phosphate pathway.The findings provide a theoretical basis for the pigment deposition in green eggshells and offer a reference for the subsequent breeding of green-shelled chickens.
Keywords：metabolomicsdifferential metabolitesgreen eggshellsXuefeng black-boned chickeneggshell color
Publication Date：2024-12-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 89-93 )