Acute toxicity study of tripyridine-zinc complex in mice

LI Lin-shen; MENG Shu-ling; CHEN Hai-xiu; DUAN Xiao-ge; LUO Jia-qi; HUANG Wei-yang; JIANG Ming-sheng; CHEN Hai-lan

doi:10.13557/j.cnki.issn1002-2813.2024.21.014

Abstract：The experiment used Kunming mice as a vehicle to conduct an acute toxicity study on the tripyridine-zinc complex(LB-ZnI2).The absolute lethal dose(LD100)and the maximum tolerable dose(LD0)of LB-ZnI2 to Kunming mice were firstly determined by the dose declining method.Coriolis method was then used to design the animal grouping method and respective dosage.The death,clinical manifestations and pathological changes of important organs of mice were recorded during the experiment period.The results showed that,the median lethal dose(LD50)and 95％confidence limits of LB-ZnI2 provided by intraperitoneal injection were 66.0 mg/kg·bw and 53.8～80.9 mg/kg·bw.When the dosage was less than or equal to 22 mg/kg·bw,no death was happened,and there was no significant difference in the morphology,color and tissue section of the main organs between the treated mice and the normal control group.However,when the dosage was greater than 33 mg/kg·bw,the animal began to die and the number of deaths increased with the increase of drug dosage.In addition,the lung of mice were found to be congested with blood and the spleen were atrophied.Histological examination revealed decreased lymphocyte necrosis in the spleen,atrophy of the splenic corpuscle,shedding of alveolar epithelial cells in the lungs,widening of the lung interstitium,and infiltration of numerous inflammatory cells with abundant red blood cells.These changes were positively correlated with the applied dose.The study indicates that,according to the acute toxicity dosage classification of chemical substances in the Technical Guidelines for Veterinary Drug Research(2006-2011 edition),LB-ZnI2 can be judged as a moderately toxic chemical,and the drug dose should be lower than 22 mg/kg·bw in subsequent chronic toxicity and pharmacodynamics studies.