Study on enzymatic hydrolysis process of non-starch polysaccharides of rice bran meal in vitro
XUAN Li
QUAN Zhi-zhong
Abstract:The experiment aims to study the effects of xylanase,cellulase,and phytase on the degradation of non-starch polysaccharides in rice bran through in vitro enzymatic pre-digestion treatment.Experiment with defatted rice bran as the raw material,using single-factor experiments and response surface methodology to optimize the process conditions for the degradation of non-starch polysaccharides in rice bran by xylanase,cellulase,and phytase.The results showed that the optimum conditions for degradation of non-starch polysaccharides in rice bran meal in vitro were:pH value 3.7,temperature 50 ℃,xylanase content 2.0%,cellulase content 1.0%,phytase content 1.8%and enzymatic hydrolysis time 3.5 h.After enzymatic hydrolysis pretreatment,the reducing sugar in rice bran meal was increased by 7 531%,inositol was increased by 1 620%,the degradation rates of neutral detergent fiber,acid detergent fiber,acid detergent lignin and crude fiber were 47.4%,24.0%,12.1%and 21.3%,respectively,the degradation rates of phytic acid was 99.9%.The study indicates that enzymatic hydrolysis predigestion technology in vitro effectively reduced the content of anti-nutrition factors in rice bran meal and improved the nutritional value of rice bran meal.
Keywords:rice bran mealnon-starch polysaccharidesxylanasecellulasephytase
Publication Date:2024-10-31
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:6( 69-74 )
